sspA

168

small acid-soluble spore protein (major alpha-type SASP)

locus: BSU_29570

Molecular weight: 6.93 kDa

pI: 4.73

Protein length: 69 aa

Gene length: 210 bp

function: protection of spore DNA

product: small acid-soluble spore protein (major alpha-type SASP)

essential: no

synonyms: sspA

List of homologs in different organisms, belongs to COG5852 (Galperin et al., 2021)

Gene

Coordinates: 3,025,445  3,025,654

Phenotypes of a mutant

• a [gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA] mutant is sensitive to ionizing radiation [Pubmed|24123749]
• a [gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA] [gene|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB] double mutant is sensitive to blue light-induced DNA damage [pubmed|30054368]

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The protein

Protein family

• [wiki|Alpha/beta-type SASP family] (according to UniProt)

Structure

• [PDB|2Z3X] ([protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|sspC] in complex with DNA, 83% identity)
• [AF|P04831]

Modification

• phosphorylated on Ser-6, Ser-9-, Ser-47, and Ser-55 [Pubmed|26381121]
• phosphorylated on Arg-55 [pubmed|31221751]

Paralogous protein(s)

• [protein|9E1B3A6289F2C94F9088FA14CE8939E208DE9FEA|sspC], [protein|AEFA4CE1588C7EC7FCB01312A172093D12B2B206|sspD], [protein|28F47ADECD376494878AE58B395B3A2616B6B5DF|sspB]

Expression and Regulation

Operons

• genes: [gene|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ]-[gene|A64A59C2B15015F13911EC53763D370F780400FD|thiI]-[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]

  description: [Pubmed|22383849]

• genes: [gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]

  description: [Pubmed|3009398]

  regulation

  • expressed late during sporulation in the forespore ([protein|search|SigG], [wiki|SpoVT]) [Pubmed|15699190,8755877]

  regulatory mechanism

  • [protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: activation, [Pubmed|8755877], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores [pubmed|30782632]

Biological materials

Mutant

• BP137 (''[gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]''::''cat'') available in [wiki|Fabian Commichau]'s lab
• 1S109 (no resistance), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S109&Search=1S109 BGSC]
• 1S111 (no resistance), [Pubmed|3087950], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S111&Search=1S111 BGSC]
• 1S112 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S112&Search=1S112 BGSC]
• 1S113 (no resistance), [Pubmed|3125155], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S113&Search=1S113 BGSC]
• BKE29570 ([gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT,  downstream forward: _UP4_TAATTTACAATTTCACATAA
• BKK29570 ([gene|80B5DAED9B4BD015D877DB4819AB25FC5F165C6D|sspA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTGTCTCACCTCCTTGT,  downstream forward: _UP4_TAATTTACAATTTCACATAA

References

Reviews

• 35638784

Original Publications

• 16707666,2456528,3009398,12884008,11092849,7608092,15458366,19542328,15699190,8755877,23064347,3087950,3125155,24123749,26381121,30054368,30782632,31221751,37949068