srfAB

168

surfactin synthetase / competence

Locus: BSU_03490

Molecular weight: 400.86 kDa

Isoelectric point: 4.9

Protein length: 3583 aa

Gene length: 10752 bp

Function: antibiotic synthesis

Product: surfactin synthetase / competence

Essential: no

Synonyms: srfAB, comL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG4908 (Galperin et al., 2021)

Gene

Coordinates: 387,744  398,495

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

• 3 Carrier domains (aa 965-1039, aa 2005-2080, aa 3034-3108) (according to UniProt)

Structure

• 2VSQ (PDB) (termination module of SrfAC, 39% identity) PubMed

Modification

• phosphorylation on Ser-999 AND Ser-2045 PubMed
• phosphorylated on Arg-1378 PubMed

Localization

• membrane PubMed

Additional information

• the protein can be degraded by adaptor-ClpP complexes PubMed

Expression and Regulation

Operons

• Genes: srfAA-srfAB-comS-srfAC-srfAD

  Description: PubMed

  Regulation

  • expressed at high cell density (ComA) PubMed
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • PhoP: activation, PubMed, in phoP regulon
  • CodY: repression, PubMed, in codY regulon
  • ComA: activation, PubMed, in comA regulon
  • PerR: activation, PubMed, in perR regulon
  • Spx: repression, PubMed, in spx regulon
  • Abh: repression, PubMed, in abh regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'HxlR' and 'SrfAA' PubMed

Biological materials

Mutant

• BKE03490 (srfAB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTTGCTCATATGCCACC,  downstream forward: _UP4_AACGAAAGCAAGGAGGAGCA
• BKK03490 (srfAB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTTTTGCTCATATGCCACC,  downstream forward: _UP4_AACGAAAGCAAGGAGGAGCA

References

Reviews

Loading

Original Publications

Loading