spoVE

168

SEDS family peptidoglycan glycosyltransferase, required for spore cortex peptidoglycan synthesis

Locus: BSU_15210

Molecular weight: 39.97 kDa

Isoelectric point: 9.85

Protein length: 366 aa

Gene length: 1101 bp

Function: spore cortex peptidoglycan synthesis

Product: SEDS family peptidoglycan glycosyltransferase

Essential: no

Synonyms: spoVE

Genomic Context

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List of homologs in different organisms, belongs to COG0772 (Galperin et al., 2021)

Gene

Coordinates: 1,590,317  1,591,417

The protein

Protein family

• SEDS proteins (shape, elongation, division, and sporulation) PubMed
• SEDS family (with FtsW and RodA, according to UniProt)

Structure

• 6BAR (PDB) (from Thermus thermophilus,corresponds to aa 99... 357, 38% identity) PubMed
• P07373 (AlphaFold)

Paralogous protein(s)

• FtsW, RodA

Localization

• integral membrane protein, forespore PubMed

Expression and Regulation

Operons

• Genes: murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

• Genes: spoVE

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

• Genes: spoVE-murG-murB-divIB-ylxW-ylxX-sbp

  Description: PubMed

  Regulation

  • expressed during vegatative growth, then switched off, and again expressed during sporulation in the mother cell (SigE) PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Mutant

• BKE15210 (spoVE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAATCGACACCCCAA,  downstream forward: _UP4_TAACGAATGTATTTCCAAGC
• BKK15210 (spoVE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAATCGACACCCCAA,  downstream forward: _UP4_TAACGAATGTATTTCCAAGC

References

Reviews

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Original Publications

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