spoVD

168

mother-cell specific class B penicillin-binding protein (spore cortex)

Locus: BSU_15170

Molecular weight: 71.08 kDa

Isoelectric point: 8.85

Protein length: 646 aa

Gene length: 1941 bp

Function: synthesis of spore peptidoglycan

Product: penicillin-binding protein (spore cortex)

Essential: no

Synonyms: spoVD

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall synthesis, Penicillin-binding proteins

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

Gene

Coordinates: 1,584,214  1,586,154

The protein

Catalyzed reaction/ biological activity

synthesis of spore peptidoglycan PubMed
protects SpoVE from proteolytic degradation PubMed

Protein family

transpeptidase family (according to UniProt)

Domains

C-terminal PASTA domain (aa 580-638) PubMed

Structure

1PYY (PDB) (PBP2B from Spreptococcus pneumoniae, 26% identity) PubMed
Q03524 (AlphaFold)

Effectors of protein activity

intramolecular disulfide bonds between two Cys residues are reduced by StoA, this is rquired for activity of SpoVD PubMed

Paralogous protein(s)

PbpB

Localization

intermembrane space that separates forespores from mother cells PubMed
outer forespore membrane PubMed

Expression and Regulation

Operons

Genes: spoVD

Description: PubMed

Regulation

expressed during sporulation in the mother cell (SigE) PubMed

Regulatory mechanism

SpoIIID: repression, PubMed, in spoIIID regulon

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

1A975 ( spoVD::cat), PubMed, available at BGSC
BKE15170 (spoVD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT,  downstream forward: _UP4_TGATTCGGGCTGCCTATTCT
BKK15170 (spoVD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT,  downstream forward: _UP4_TGATTCGGGCTGCCTATTCT

References

Reviews

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Original Publications

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