spoVAF

168

part of an ion channel for spore germination, amplifies the response to spore germinants

Locus: BSU_23390

Molecular weight: 55.44 kDa

Isoelectric point: 6.21

Protein length: 493 aa

Gene length: 1482 bp

Function: spore germination

Product: part of the SpoVAF-FigP heteromeric ion channel

Essential: no

Synonyms: spoVAF

Genomic Context

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List of homologs in different organisms, belongs to COG5840 (Galperin et al., 2021)

Gene

Coordinates: 2,438,372 → 2,439,853

Phenotypes of a mutant

• delayed germination with germinant receptor-dependent germinants PubMed

The protein

Catalyzed reaction/ biological activity

• the SpoVAF-FigP ion channel releases K⁺ under high pressure conditions PubMed

Protein family

• GerABKA family (according to UniProt)

Structure

• P31845 (AlphaFold)

Localization

• integral inner spore membrane protein PubMed

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: activation, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• BKE23390 (ΔspoVAF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACGGTTTTTGAAATACTCTT,  downstream forward: _UP4_TAATCGCATTGAAACTGACT
• BKK23390 (ΔspoVAF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACGGTTTTTGAAATACTCTT,  downstream forward: _UP4_TAATCGCATTGAAACTGACT

References

Reviews

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Original Publications

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