spoVAEA

168

stage V sporulation germinant protein, essential for the transport of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 )

Locus: BSU_23401

Molecular weight: 22.00 kDa

Isoelectric point: 5

Protein length: 203 aa

Gene length: 612 bp

Function: spore germination

Product: stage V sporulation germinant protein

Essential: no

Synonyms: spoVAEA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5838 (Galperin et al., 2021)

Gene

Coordinates: 2,439,804 → 2,440,415

Phenotypes of a mutant

• delayed germination with germinant receptor-dependent germinants PubMed

The protein

Structure

• P40870 (AlphaFold)

Localization

• outer surface of the spore's inner membrane PubMed

Expression and Regulation

Operons

• Genes: spoVAA-spoVAB-spoVAC-spoVAD-spoVAEB-spoVAEA-spoVAF-lysA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: activation, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• BKE23401 (ΔspoVAEA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTGCGTTTTGTCGTCATGT,  downstream forward: _UP4_TTTATTTTAGAAAGGAGCGG
• BKK23401 (ΔspoVAEA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTGCGTTTTGTCGTCATGT,  downstream forward: _UP4_TTTATTTTAGAAAGGAGCGG

Labs working on this gene/protein

• Peter Setlow, University of Connecticut Health Center, USA

References

Reviews

Loading

Original Publications

Loading