spoIVFB

168

intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active SigK

Locus: BSU_27970

Molecular weight: 33.49 kDa

Isoelectric point: 8.48

Protein length: 288 aa

Gene length: 867 bp

Function: processing of pro-sigma-K to active SigK

Product: intramembrane metalloprotease

Essential: no

Synonyms: spoIVFB

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,855,973  2,856,839

The protein

Catalyzed reaction/ biological activity

• processing of pro-sigma-K to active SigK PubMed

Protein family

• peptidase M50B family (according to UniProt)

Domains

• C-terminal CBS domain, this domain binds ATP PubMed

Cofactors

• Zn²⁺ PubMed

Structure

• 8VJL (PDB) (SpoIVFB-Pro-SigK) PubMed
• P26937 (AlphaFold)

Effectors of protein activity

• ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level PubMed
• SpoIVFB is kept inactive by the interaction with SpoIVFA and BofA PubMed

Localization

• mother cell integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: spoIVFA-spoIVFB

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

  Additional information

  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

• Genes: spoIVFA-spoIVFB-rplU-prp-rpmA

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Additional information

  • the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Biological materials

Mutant

• BKE27970 (spoIVFB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA,  downstream forward: _UP4_TAAAACTGATTGACAAACGC
• BKK27970 (spoIVFB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA,  downstream forward: _UP4_TAAAACTGATTGACAAACGC

References

Reviews

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Original Publications

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