spoIVFA

168

inhibitor of SpoIVFB metalloprotease

Locus: BSU_27980

Molecular weight: 29.46 kDa

Isoelectric point: 7.29

Protein length: 264 aa

Gene length: 795 bp

Function: control of SigK activation

Product: inhibitor of SpoIVFB metalloprotease

Essential: no

Synonyms: spoIVFA, bofB, spoVL

Genomic Context

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Categories containing this gene/protein

Information processing, Regulation of gene expression, Sigma factors and their control, Control of sigma factors

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

Groups of genes, Membrane proteins

List of homologs in different organisms, belongs to COG5833 (Galperin et al., 2021)

Gene

Coordinates: 2,856,832  2,857,626

The protein

Domains

the ectodomain of SpoIVFA is cleaved off by SpoIVB (first cleavage) PubMed
the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Structure

P26936 (AlphaFold)

Localization

integral mother cell membrane protein PubMed

Expression and Regulation

Operons

Genes: spoIVFA-spoIVFB

Description: PubMed

Regulation

expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

Regulatory mechanism

SpoIIID: repression, PubMed, in spoIIID regulon

Sigma factors

SigE: sigma factor, PubMed, in sigE regulon

Additional information

the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Genes: spoIVFA-spoIVFB-rplU-prp-rpmA

Description: PubMed

Regulation

expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

Additional information

the domains that inhibits SpoIVFB is cleaved off by CtpB (second cleavage) PubMed

Biological materials

Mutant

BKE27980 (spoIVFA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCCATCATCCTTTGCA,  downstream forward: _UP4_ATTGATCCGATTCAGGTGAT
BKK27980 (spoIVFA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCCATCATCCTTTGCA,  downstream forward: _UP4_ATTGATCCGATTCAGGTGAT

References

Reviews

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Original Publications

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