spoIIM

168

required for dissolution of the septal cell wall

locus: BSU_23530

Molecular weight: 24.15 kDa

pI: 9.84

Protein length: 214 aa

Gene length: 645 bp

function: dissolution of the septal cell wall

product: sporulation protein

essential: no

synonyms: spoIIM

List of homologs in different organisms, belongs to COG1300 (Galperin et al., 2021)

Gene

Coordinates: 2,450,403  2,451,047

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The protein

Structure

• [AF|P37873]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)
• the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]
• localization of the [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]-[protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]-[protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] complex to the septal membrane depends on [protein|D98D61CA651AAA415386B1B2B41562E5CF77DEF1|spoIIB] [pubmed|36066101]

Expression and Regulation

Operons

• genes: [gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]

  description: [Pubmed|8501065]

  regulation

  • expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8501065]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8501065], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• MGNA-C416 (spoIIM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2414 NBRP B. subtilis, Japan]
• BKE23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC,  downstream forward: _UP4_TAAAATCATTATTAAAAGAA
• BKK23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23530 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC,  downstream forward: _UP4_TAAAATCATTATTAAAAGAA

References

Reviews

• 32660383,33651833,35638784

Original Publications

• 17376078,11886548,15752199,8501065,15383836,23859254,20382772,12502745,31282858,36066101