yidC1

168

Sec-independent membrane protein translocase, essential for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activity at stage III, involved in the assembly of the [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] complex

locus: BSU_41040

Molecular weight: 29.37 kDa

pI: 9.99

Protein length: 261 aa

Gene length: 786 bp

function: membrane insertion of proteins and [wiki|protein secretion]

product: membrane protein translocase

essential: no

synonyms: yidC1, spoIIIJ, undefined, spo0J87

List of homologs in different organisms, belongs to COG0706 (Galperin et al., 2021)

Gene

Coordinates: 4,213,823 → 4,214,608

Phenotypes of a mutant

• defective in sporulation [pubmed|1487728]

additional information

• a [protein|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1] gain-of-function mutation (Q140K) is present in B. subtilis strain PY97 [pubmed|31626625]

Advertisement

The protein

Catalyzed reaction/ biological activity

• membrane protein translocase, facilitates insertion of [protein|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE] into the membrane [Pubmed|18485064]

Protein family

• OXA1/ALB3/YidC family (with [protein|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2], according to UniProt) [Pubmed|20800571,21194367,15802250]

Structure

• [PDB|3WO6] (from ''Bacillus halodurans'', 51% identity) [Pubmed|24739968]
• [AF|Q01625]

Paralogous protein(s)

• [protein|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2], one of the two proteins has to be present for viability [Pubmed|17114254]

[wiki|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|EB07AECD3849CC0B65126DE27E71B28B3AD106B0|rpmH]-[gene|594F2D36BE71A45303A33AA2A2931F56F0A969ED|rnpA]-[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]

  description: [Pubmed|22383849]

  regulation

  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

• genes: [gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]-[gene|23D33E2D7AB1CA02165070DB8238957E3C768E64|jag]

  description: [Pubmed|1487728]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1487728], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • An antisense RNA is predicted for '[protein|search|jag]' [PubMed|20525796]

Biological materials

Mutant

• BKE41040 (Δ[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE41040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCCTATAATTAAT,  downstream forward: _UP4_AAGTGAGGAATGTGACTGCT
• BKK41040 (Δ[gene|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK41040 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCCTATAATTAAT,  downstream forward: _UP4_AAGTGAGGAATGTGACTGCT

labs

• [wiki|Richard Losick], Harvard University, Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
• [wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]

References

Reviews

• 22688815,20800571,21194367,15802250,25947384,27879020,35638784

Original Publications

• 18485064,12813085,18820020,1487728,23852076,18763711,17114254,11889108,15995216,12586834,19717609,19779460,22864117,24443530,25133632,25313395,25359772,25855636,26331454,24739968,31002900,31626625,35148995,37290739,38474341