sndB

168

N-acetylcysteine deacetylase

locus: BSU_39470

Molecular weight: 41.44 kDa

pI: 5.32

Protein length: 380 aa

Gene length: 1143 bp

function: utilization and detoxification of S-(2-succino)cysteine

product: N-acetylcysteine deacetylase

essential: no

synonyms: sndB, yxeP

List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)

Gene

Coordinates: 4,056,870 → 4,058,012

Phenotypes of a mutant

• a ''[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA] [gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB] [gene|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC]'' triple mutant does not grow with N-acetyl cysteine and S-(2-succino)cysteine as the single sources of sulfur [Pubmed|23944997,29626092]

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The protein

Catalyzed reaction/ biological activity

• N-acetylcysteine + oxaloacetate --> cysteine + acetate [Pubmed|29626092]

Protein family

• [wiki|peptidase M20 family] (according to UniProt)

Structure

• [PDB|1YSJ]
• [AF|P54955]

Paralogous protein(s)

• [protein|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|ykuR], [protein|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA], [protein|AE824A62089631F129C3AA469B6ACA71DC13D2F8|amhX]
• [protein|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC], (38,8%)

Expression and Regulation

Operons

• genes: [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]

  description: [Pubmed|10746760]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B712 (yxeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1711 NBRP B. subtilis, Japan]
• BKE39470 (Δ[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC,  downstream forward: _UP4_GTTATTGTATTGGAGACCAT
• BKK39470 (Δ[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GTCGGCCATTGTTCTTCTCC,  downstream forward: _UP4_GTTATTGTATTGGAGACCAT

References

• 10746760,23944997,16513748,29626092