snaB

168

S-(2-succino)cysteine N-acetyltransferase

locus: BSU_39510

Molecular weight: 18.88 kDa

pI: 9.12

Protein length: 165 aa

Gene length: 498 bp

function: utilization and detoxification of S-(2-succino)cysteine

product: S-(2-succino)cysteine N-acetyltransferase

essential: no

synonyms: snaB, yxeL

List of homologs in different organisms, belongs to COG0456 (Galperin et al., 2021)

Gene

Coordinates: 4,060,307 → 4,060,804

Phenotypes of a mutant

• the ''[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA] [gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]'' double mutant does not grow with S-methyl cysteine [Pubmed|23944997]
• no growth with S-(2-succino)cysteine as the single source of sulfur [pubmed|29626092]
• resistant to S-(2-succino)cysteine toxicity [pubmed|29626092]

RNA

Catalyzed reaction/ biological activity

• acetylation of S-(2-succino)cysteine [pubmed|29626092]

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The protein

Protein family

• [wiki|Acetyltransferase family] (according to UniProt)

[wiki|Domains]

• [wiki|N-acetyltransferase domain] (aa 3-162) (according to UniProt)

Structure

• [AF|P54951]

Paralogous protein(s)

• [protein|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]

Expression and Regulation

Operons

• genes: [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]

  description: [Pubmed|10746760]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B781 (yxeL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1780 NBRP B. subtilis, Japan]
• BKE39510 (Δ[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCGTGGCTTCATCTAGCGTT,  downstream forward: _UP4_TAAAAATAGAAAAGGCGGGA
• BKK39510 (Δ[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TCGTGGCTTCATCTAGCGTT,  downstream forward: _UP4_TAAAAATAGAAAAGGCGGGA

References

• 10746760,15937167,16513748,21815947,23944997,29626092