sfp/2

168

4-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168

locus: BSU_03570

Protein length: 165 aa

Gene length: 498 bp

function: phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits of surfactin synthetase (SrfAA-SrfAB-SrfAC) and AcpK

product: 4-phosphopantetheinyl transferase

essential: no

ec: null

synonyms: sfp\/2, sfp

List of homologs in different organisms

Gene

Coordinates: 407,638  408,135

Phenotypes of a mutant

• No swarming motility on B medium. [Pubmed|19202088]

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The protein

Catalyzed reaction/ biological activity

• CoA + apo-[peptidyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] (according to Swiss-Prot)

Protein family

• P-Pant transferase superfamily (with [protein|200BE4802FCE3C860565B374EAADC9C1FEEF3E49|acpS], according to UniProt)

Structure

• [PDB|4MRT] [pubmed|24704508]

Biological materials

Mutant

• BKE03570 ([gene|1627167E7E9DBE444161A94D9F76317612C6401C|sfp/2]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTAGATCCTCCGTCTG,  downstream forward: _UP4_ATCGCTTCCGCTTGATTCCT
• BKK03570 ([gene|1627167E7E9DBE444161A94D9F76317612C6401C|sfp/2]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03570 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTAGATCCTCCGTCTG,  downstream forward: _UP4_ATCGCTTCCGCTTGATTCCT

labs

• [wiki|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 20735481,24704508

Original Publications

• 15955059,19202088,2848009,11489886,15065855,10581256,15066026,11867633,19202088,19749039,20094656,9484229,20148996,21278284,21466506,37243871