sdpC

168

toxin, collapses the proton motive force and induces autolysis, kills non-sporulating cells, induces activity of SigW

Locus: BSU_33770

Molecular weight: 22.07 kDa

Isoelectric point: 9.61

Protein length: 203 aa

Gene length: 612 bp

Function: killing of non-sporulating sister cells

Product: toxin, kills non-sporulating cells

Essential: no

Synonyms: sdpC, yvaY

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,465,776  3,466,387

The protein

Catalyzed reaction/ biological activity

• toxin, collapses the proton motive force and induces autolysis PubMed

Structure

• O34344 (AlphaFold)

Modification

• the mature SDP is a 42-residue peptide with one disulfide bridge PubMed

Paralogous protein(s)

• YitM

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: sdpA-sdpB-sdpC

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon
  • Rok: repression, PubMed, in rok regulon
  • AbrB: repression, PubMed, in abrB regulon

  Additional information

  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-A452 (yvaY::erm), available at the NBRP B. subtilis, Japan
• BKE33770 (sdpC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTATTATACCTCCATT,  downstream forward: _UP4_TAATCCATTATAATTGAGTG
• BKK33770 (sdpC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTATTATACCTCCATT,  downstream forward: _UP4_TAATCCATTATAATTGAGTG

References

Reviews

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Original Publications

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