sdaAA

168

L-serine deaminase

locus: BSU_15860

Molecular weight: 30.78 kDa

pI: 4.87

Protein length: 300 aa

Gene length: 903 bp

function: serine utilization

product: L-serine deaminase (alpha chain)

essential: no

ec: 4.3.1.17

synonyms: sdaAA, ylpA, sdaA

List of homologs in different organisms, belongs to COG1760 (Galperin et al., 2021)

Gene

Coordinates: 1,658,930  1,659,832

Phenotypes of a mutant

• delayed [wiki|biofilm formation] [pubmed|31138626]
• overexpression of [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA] results in resistance to serine [pubmed|32743959]

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The protein

Catalyzed reaction/ biological activity

• L-serine --> NH₄⁺ + pyruvate (according to UniProt)

Protein family

• iron-sulfur dependent L-serine dehydratase family (with [protein|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB], according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [Pubmed|22686449]

Structure

• [AF|O34607]

Expression and Regulation

Operons

• genes: [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE15860 ([gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACTGGCTCCTCCTGAC,  downstream forward: _UP4_ATTTTCGGAGGAGCGCTAGG
• BKK15860 ([gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAACTGGCTCCTCCTGAC,  downstream forward: _UP4_ATTTTCGGAGGAGCGCTAGG

References

• 22383849,22686449,24161940,31138626,32743959