scoB

168

succinyl CoA:3-oxoacid CoA-transferase (subunit B)

locus: BSU_38980

Molecular weight: 23.18 kDa

pI: 4.89

Protein length: 216 aa

Gene length: 651 bp

function: 3-hydroxybutyrate utilization

product: succinyl CoA:3-oxoacid CoA-transferase (subunit B)

essential: no

ec: 2.8.3.5

synonyms: scoB, yxjE

List of homologs in different organisms, belongs to COG2057 (Galperin et al., 2021)

Gene

Coordinates: 4,001,329  4,001,979

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The protein

Catalyzed reaction/ biological activity

• 3-oxo acid + succinyl-CoA --> 3-oxoacyl-CoA + succinate (according to UniProt)

Protein family

• 3-oxoacid CoA-transferase subunit B family (with [protein|138832210AC94B17E081F3D0AF9BCFE3BE63E601|yodR], according to UniProt)

Structure

• [PDB|3CDK] (the [protein|A7E61C3D12BBDDD3EFD65B1EDBE280497D7B6CD0|scoA]-[protein|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB] complex)
• [AF|P42316]

Paralogous protein(s)

• [protein|138832210AC94B17E081F3D0AF9BCFE3BE63E601|yodR]

Biological materials

Mutant

• MGNA-B729 (yxjE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1728 NBRP B. subtilis, Japan]
• BKE38980 ([gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTCACTTGGCCTCACCCTT,  downstream forward: _UP4_AATTCTTAATGGGAAGGTGT
• BKK38980 ([gene|7F46EDA8F1EB8D9817C62D7D361FF32F9CD814AD|scoB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTTCACTTGGCCTCACCCTT,  downstream forward: _UP4_AATTCTTAATGGGAAGGTGT

References

• 17363272,10746760,16672620,12662922,12850135,10666464