sbcE

168

SMC-like protein, involved in DNA double-strand break repair and competence

locus: BSU_09920

Molecular weight: 110.96 kDa

pI: 5.79

Protein length: 963 aa

Gene length: 2892 bp

function: DNA double-strand break repair and competence

product: SMC-like protein

essential: no

synonyms: sbcE, yhaN

List of homologs in different organisms, belongs to COG4717 (Galperin et al., 2021)

Gene

Coordinates: 1,066,077  1,068,968

Phenotypes of a mutant

• sensitive to DNA damage [pubmed|19906728]

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The protein

Structure

• [AF|O08455]

[wiki|Localization]

• Membrane-proximal (Spotty) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|3E27C881BB3DE3FD55B7AD21B8771A859D80786F|yhaO]-[gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]-[gene|6D585EB5E56A1E5C1C31680D960043BA08C817E2|yhaM]

  description: [Pubmed|16267290]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• GP3528 (Δ[gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]::spec), available in [wiki|Jörg Stülke]'s lab
• MGNA-A663 (yhaN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/663 NBRP B. subtilis, Japan]
• BKE09920 ([gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATAATTCGCAAAGCTGTCA,  downstream forward: _UP4_TAAAAGAGGTTCTATAGCTG
• BKK09920 ([gene|709C4D0AE9C78C9A367F37B9C3B37D2516850D7C|sbcE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09920 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TATAATTCGCAAAGCTGTCA,  downstream forward: _UP4_TAAAAGAGGTTCTATAGCTG

References

• 16267290,16479537,19903937,19906728