ruvB

168

Holliday junction DNA helicase, part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] branch migration translocase and of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome

locus: BSU_27730

Molecular weight: 37.31 kDa

pI: 5.15

Protein length: 334 aa

Gene length: 1005 bp

function: [wiki|DNA repair/ recombination]

product: Holliday junction DNA helicase

essential: no

synonyms: ruvB

List of homologs in different organisms, belongs to COG2255 (Galperin et al., 2021)

Gene

Coordinates: 2,835,150  2,836,154

Phenotypes of a mutant

• a [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] mutant is not viable [pubmed|16020779]
• the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|24770420]
• the inactivations of ''[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal  [pubmed|28527403]

The protein

Catalyzed reaction/ biological activity

• [protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-ATP stimulates [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-mediated Holliday junction resolution [Pubmed|24770420]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• ruvB family (single member, according to UniProt)

Structure

• [PDB|1IN4] (from Thermotoga maritima, 57% identity) [pubmed|11478862]
• [AF|O32055]

Effectors of protein activity

• [protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] [pubmed|34768753]

[wiki|Interactions]

• part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome [pubmed|24770420]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[gene|6CDF1836F5B613A6718308A4A27D713193EA197B|yrzS]-[gene|23174B2A19DEDF2F20858A005D0681D36E15E6E3|queA]-[gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt]-[gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF]

  description: [pubmed|22383849]

  regulation

  • the mRNA is processed between [gene|0383C8214ADE2FF70CFDB953A65648B37A9D3216|tgt] and [gene|D15B74263167DAD3FA9B7384B5AA4897EAD498F2|yrbF] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]

• genes: [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]

  description: [Pubmed|16267290]

  regulation

  • induced upon DNA damage ([protein|search|LexA]) [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

Biological materials

Mutant

• GP3530 (Δ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB])::kan), available in [wiki|Jörg Stülke]'s lab
• 1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]
• BKE27730 ([gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC
• BKK27730 ([gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC

References

Reviews

• 22933559,9442895,38052445,40874739

Original Publications

• 16267290,24770420,16020779,11478862,28527403,21600217,34768753