rpsU

168

ribosomal protein bS21

locus: BSU_25410

Molecular weight: 6.69 kDa

pI: 11.58

Protein length: 57 aa

Gene length: 174 bp

function: translation

product: ribosomal protein S21 (bS21)

essential: no

synonyms: rpsU, yqeX

List of homologs in different organisms, belongs to COG0828 (Galperin et al., 2021)

Gene

Coordinates: 2,620,371  2,620,544

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bS21 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|P21478]

Additional information

• this ribosomal protein is lacking in some bacteria [pubmed|33753464]

Expression and Regulation

Operons

• genes: [gene|4C79DEC2524735E3D32F967D511C5B0761D503A5|rpsU]-[gene|ED016475DF70DC63EE97B9FFD0CAC026E13A10E3|yqeY]

  description: [pubmed|22383849]

  regulation

  • expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]

  expression

  • expression is increased upon depletion of depletion of tRNA maturation factors [gene|57A79AAF00243B5E058FA901D43AA7B55CA33F63|rnpB] or [gene|C40C5D35ED53D343C8200248FCCB010BAB388054|rnz] [pubmed|36840557]

Biological materials

Mutant

• BKE25410 ([gene|4C79DEC2524735E3D32F967D511C5B0761D503A5|rpsU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTTTCCCTCCCTCC,  downstream forward: _UP4_AAATTCTAAAAGAGGGTGGA
• BKK25410 ([gene|4C79DEC2524735E3D32F967D511C5B0761D503A5|rpsU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTCTTTCCCTCCCTCC,  downstream forward: _UP4_AAATTCTAAAAGAGGGTGGA

References

• 36815589,19653700,23002217,25035996,25903689,28516784,33753464,33782055,36840557