rpoY

168

epsilon subunit of [wiki|RNA polymerase]

locus: BSU_14540

Molecular weight: 8.12 kDa

pI: 4.61

Protein length: 69 aa

Gene length: 210 bp

function: control of [wiki|RNA polymerase] activity

product: epsilon subunit of [wiki|RNA polymerase]

essential: no

synonyms: rpoY, ykzG

List of homologs in different organisms, belongs to COG5503 (Galperin et al., 2021)

Gene

Coordinates: 1,524,791  1,525,000

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The protein

Protein family

• UPF0356 family (single member, according to UniProt)

Structure

• [PDB|4NJC] [Pubmed|25092033]
• [AF|O31718]

[wiki|Localization]

• colocalizes with the nucleoid [Pubmed|25092033]

Expression and Regulation

Operons

• genes: [gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]-[gene|3EB289D0F58A58C693AB588798EE66A731341999|rnjA]

  description: [Pubmed|24187087]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24187087], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA,  downstream forward: _UP4_AAAGTATTGGAGTTATGAGC
• BKK14540 ([gene|5F41273FB867C688011738CD45AA6FF5D3987EFE|rpoY]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCAAATCTCTCCTTAA,  downstream forward: _UP4_AAAGTATTGGAGTTATGAGC

Expression vectors

• pGP2824: expression in ''B. subtilis'', with C-terminal Strep-tag, in [wiki|pGP382], available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• pGP420 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 25878038,34403307

Original Publications

• 36815589,24187087,20724389,25092033