rpoA

168

RNA polymerase alpha subunit

Locus: BSU_01430

Molecular weight: 34.64 kDa

Isoelectric point: 4.59

Protein length: 314 aa

Gene length: 945 bp

Function: transcription

Product: RNA polymerase alpha subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms: rpoA

Genomic Context

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List of homologs in different organisms, belongs to COG0202 (Galperin et al., 2021)

Gene

Coordinates: 148,931 149,875

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ribonucleoside 5'-triphosphate + RNA_(n) --> diphosphate + RNA_(n+1)(according to UniProt)

Protein family

• RNA polymerase alpha chain family (single member, according to UniProt)

Structure

• 6WVJ (PDB) (RNA polymerase elongation complex, RpoA-RpoB-RpoC-YloH) PubMed
• 1Z3E (PDB) (C-terminal domain, complex with Spx)
• 3IYD (PDB) (RNA polymerase from E. coli, 46% identity) PubMed
• P20429 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

References

Reviews

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Original Publications

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