rpmGA

168

ribosomal protein bL33a

Locus: BSU_24900

Molecular weight: 5.85 kDa

Isoelectric point: 10.44

Protein length: 49 aa

Gene length: 150 bp

Function: translation

Product: ribosomal protein L33a (bL33a)

Essential: no

Synonyms: rpmGA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0267 (Galperin et al., 2021)

Gene

Coordinates: 2,574,408 → 2,574,557

The protein

Protein family

• bacterial ribosomal protein bL33 family (with RpmGB and RpmGC, according to UniProt)

Cofactors

• requires zinc for activity PubMed

Structure

• 3J9W (PDB) (the ribosome) PubMed
• P56849 (AlphaFold)

Modification

• phosphorylated on Arg-29 PubMed

Paralogous protein(s)

• RpmGB, RpmGC

Localization

• ribosome (according to UniProt)

Additional information

• the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed

Expression and Regulation

Operons

• Genes: rpmGA

  Description: PubMed

  Regulation

  • expression is reduced in motile cells as compared to non-motile cells PubMed

• Genes: yqgL-yqgM-rpmGA

  Description: PubMed

Biological materials

Mutant

• BKE24900 (ΔrpmGA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTTCCCTCCAAACT,  downstream forward: _UP4_TAACAGCTTTGCTGTTTGAG
• BKK24900 (ΔrpmGA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTATTTTCCCTCCAAACT,  downstream forward: _UP4_TAACAGCTTTGCTGTTTGAG

References

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