rpmF

168

ribosomal protein bL32

locus: BSU_15080

Molecular weight: 6.59 kDa

pI: 10.77

Protein length: 59 aa

Gene length: 180 bp

function: translation

product: ribosomal protein L32 (bL32)

essential: no

synonyms: rpmF

List of homologs in different organisms, belongs to COG0333 (Galperin et al., 2021)

Gene

Coordinates: 1,575,804  1,575,983

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The protein

Protein family

• bacterial [wiki|ribosomal protein] bL32 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
• [AF|O34687]

Additional information

• this ribosomal protein is lacking in some organisms with very small genomes [pubmed|33753464]

Expression and Regulation

Operons

• genes: [gene|AF4BB5F08F805D3621597559B4C7F53A1A1CAD05|ylbN]-[gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]

  description: [Pubmed|22383849]

  regulation

  • strongly repressed in response to glucose starvation in M9 medium [Pubmed|23033921]

  regulatory mechanism

  • stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]

Biological materials

Mutant

• BKE15080 ([gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTCCCACCTCCTTAA,  downstream forward: _UP4_TAATGAGAAAAGCGCAGGGT
• BKK15080 ([gene|8C16300FC29E7DB5070367C8A2568134AF528233|rpmF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTCCCACCTCCTTAA,  downstream forward: _UP4_TAATGAGAAAAGCGCAGGGT

References

• 36815589,19653700,22383849,11948165,23002217,23033921,25903689,33753464