rplK

168

ribosomal protein uL11, recruitment of RqcH to the 50S subunit of stalled ribosomes

Locus: BSU_01020

Molecular weight: 14.78 kDa

Isoelectric point: 9.72

Protein length: 141 aa

Gene length: 426 bp

Function: translation

Product: ribosomal protein L11 (uL11)

Essential: no

Synonyms: rplK, relC, tsp

Genomic Context

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List of homologs in different organisms, belongs to COG0080 (Galperin et al., 2021)

Gene

Coordinates: 118,591 → 119,016

Phenotypes of a mutant

• poor growth PubMed
• non-transformable PubMed
• defective in ribosome quality control PubMed
• inactivation of rplK confers resistance to thiopeptide antibiotics PubMed

The protein

Catalyzed reaction/ biological activity

• recruitment of RqcH to the 50S subunit of stalled ribosomes PubMed

Protein family

• Universal ribosomal protein uL11 family (single member, according to UniProt)

Structure

• 2FOW (PDB) (RNA binding domain in complex with RNA, Geobacillus stearothermophilus) PubMed
• 1FOX (PDB) (C-terminal domain, Geobacillus stearothermophilus) PubMed
• 3J9W (PDB) (the ribosome) PubMed
• Q06796 (AlphaFold)

Modification

• phosphorylated on Arg-94 PubMed

Localization

• ribosome (according to UniProt)

Expression and Regulation

Operons

• Genes: rplK-rplA-BSU_misc_RNA_3-rplJ-rplL

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

Biological materials

Mutant

• BKE01020 (ΔrplK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGAGACACACCTCCTTAA,  downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT
• BKK01020 (ΔrplK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGAGACACACCTCCTTAA,  downstream forward: _UP4_TAATTTGTTTCTTGTCGGGT

References

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