rplA

rplA
168

ribosomal protein uL1

locus
BSU_01030
Molecular weight
24.77 kDa
pI
9.73
Protein length
Gene length
function
translation
product
ribosomal protein L1 (uL1)
essential
no
synonyms
rplA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0081 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
119,111  119,809
Phenotypes of a mutant
slow growth, this can be suppressed by inactivation of [gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA] [pubmed|29967120]
reduced sporulation frequency, this can be suppressed by inactivation of [gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA] [pubmed|29967120]
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The protein
Protein family
universal [wiki|ribosomal protein] uL1 family (single member, according to UniProt)
Structure
[PDB|3J9W] (the [wiki|ribosome]) [Pubmed|25903689]
[AF|Q06797]
[wiki|Localization]
[wiki|ribosome] (according to UniProt)
cytoplasm, region surrounding the nucleoid [pubmed|36406443]
Additional information
this ribosomal protein is lacking in some organisms with very small genomes [pubmed|33753464]
Expression and Regulation
Operons
genes
[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]-[gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]-[gene|E4D89640D0F0A31A2814D20FB5425FDB0B46A43D|BSU_misc_RNA_3]-[gene|5EF3A7953AA97D2B44C967542A53FEA7F36D7BA3|rplJ]-[gene|E72910604D3DC2A62AE8A951F55570EA2838B250|rplL]
description
[pubmed|22383849]
regulation
[protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, [pubmed|11948165], in [regulon|other_regulator:stringent response|stringent response]
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[gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]->[gene|E72910604D3DC2A62AE8A951F55570EA2838B250|rplL]

2024-03-16 02:38:50

Jstuelk

60

a3b77f9cd37eef4d41bc80e15b9131374c283aa8

9D93F55A2AB271535602470E50EFA15E2E175400

Biological materials
Mutant
BKE01030 ([gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT,  downstream forward: _UP4_TAATATTGACATGACATCAA
BKK01030 ([gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01030 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT,  downstream forward: _UP4_TAATATTGACATGACATCAA
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1094 (erm, based on [wiki|pGP1087]), available in [wiki|Jörg Stülke]'s lab
References
17981968,19653700,11463749,22848659,23175651,23002217,25903689,29967120,33753464,36406443

44D4CE353E1E532664A508CBE8B49AC8BB861B53

Page visits: 5606

Time of last update: 2024-03-28 14:09:11

Author of last update: Jstuelk