rocF

168

arginase

locus: BSU_40320

Molecular weight: 32.00 kDa

pI: 4.93

Protein length: 296 aa

Gene length: 891 bp

function: arginine utilization

product: arginase

essential: no

ec: 3.5.3.1

synonyms: rocF, RocF

List of homologs in different organisms, belongs to COG0010 (Galperin et al., 2021)

Gene

Coordinates: 4,141,711 → 4,142,601

Phenotypes of a mutant

• unable to grow with arginine as single source of nitrogen [pubmed|37343703]

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The protein

Catalyzed reaction/ biological activity

• H₂O + L-arginine --> L-ornithine + urea (according to UniProt)

Protein family

• arginase family (with [protein|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB] and [protein|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG], according to UniProt)

[wiki|Cofactors]

• Mn²⁺ [pubmed|34160574]

Structure

• [PDB|6NFP]
• [PDB|6DKT]
• [AF|P39138]

Modification

• phosphorylated on Ser-68 [Pubmed|20509597]

Kinetic information

• Km for arginine: 4.6 mM [pubmed|23625224]

Biological materials

Mutant

• GP655, aphA3, available in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
• BKE40320 (Δ[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
• BKK40320 (Δ[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC

Expression vectors

• pGP3796: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
• pGP3780: expression of Strep-''rocF'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 34160574

Original Publications

• 12618455,20509597,14651647,12618455,7540694,23625224,10196128,4216455,35787694,35994323,37343703