rnr

168

RNase R, required for protection against paraquate stress

Locus: BSU_33610

Molecular weight: 88.56 kDa

Isoelectric point: 5.7

Protein length: 779 aa

Gene length: 2340 bp

Function: nonspecific degradation of rRNA

Product: exoribonuclease RNase R (EC 3.1.-.-)

Essential: no

Synonyms: rnr, yvaJ

Genomic Context

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List of homologs in different organisms, belongs to COG0557 (Galperin et al., 2021)

Gene

Coordinates: 3,451,863  3,454,202

Phenotypes of a mutant

• defective in Swarming motility (prolonged period of immotility prior to Swarming) PubMed

The protein

Catalyzed reaction/ biological activity

• 3'-5'-exoribonuclease
• Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (according to UniProt)

Protein family

• RNR ribonuclease family (single member, according to UniProt)

Domains

• S1 domain (aa 628-708) (according to UniProt)
• 2 CSD domains (aa 73-133, aa 138-202) (according to the Interpro database)

Structure

• 5XGU (PDB) (from E. coli, 40% identity) PubMed
• O32231 (AlphaFold)

Expression and Regulation

Operons

• Genes: secG-yvaK-rnr-smpB-ssrA

  Description: PubMed

  Regulation

  • ''yvaK'': induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

• Genes: yvaK-rnr-smpB-ssrA

  Description: PubMed

  Regulation

  • ''yvaK'': induced by stress (SigB) PubMed

Biological materials

Mutant

• MGNA-A495 (yvaJ::erm), available at the NBRP B. subtilis, Japan
• BKE33610 (rnr::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG,  downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT
• BKK33610 (rnr::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCATTATCCGACCTCCTG,  downstream forward: _UP4_TAGCAGCTCAACCAGCAAAT

Labs working on this gene/protein

• David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

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Original Publications

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