rluB

168

ribosomal large subunit pseudouridine synthase B

locus: BSU_23160

Molecular weight: 25.88 kDa

pI: 10.25

Protein length: 244 aa

Gene length: 735 bp

function: 23S rRNA modification

product: pseudouridine synthase

essential: no

ec: 5.4.99.22

synonyms: rluB, ypuL

List of homologs in different organisms, belongs to COG1187 (Galperin et al., 2021)

Gene

Coordinates: 2,421,438  2,422,172

Advertisement

The protein

Catalyzed reaction/ biological activity

• uridine²⁶⁰⁵ in 23S rRNA --> pseudouridine²⁶⁰⁵ in 23S rRNA (according to E. coli RluB)

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RsuA family (with [protein|69052B24B577ABA4B20E610F679154DC97EFD8DE|ytzG], according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 2-68) (according to UniProt)

Structure

• [PDB|4LAB] (from ''E. coli'', 40% identity) [Pubmed|24214967]
• [AF|P35159]

Expression and Regulation

Operons

• genes: [gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]-[gene|F7D869F77E2275110737EF658C58FA1BF742D73F|resA]-[gene|495721E4B8BF6FEC01E62E86339560F90776EED1|resB]-[gene|97FE84CF968596AC39D19EE2E43DA625147FD702|resC]-[gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]-[gene|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A106 (ypuL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/106 NBRP B. subtilis, Japan]
• BKE23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA
• BKK23160 ([gene|5CD1E266B0EC82546A2AF19A05490DD4D04F812C|rluB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23160 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTCTTTCACCTCAAGTA,  downstream forward: _UP4_TAAATTTCACATAACCTTCA

References

• 36815589,9888802,24214967