ribE

168

riboflavin synthase (alpha subunit)

Locus: BSU_23270

Molecular weight: 23.33 kDa

Isoelectric point: 5.84

Protein length: 215 aa

Gene length: 648 bp

Function: riboflavin biosynthesis

Product: riboflavin synthase (alpha subunit)

Essential: no

E.C.: 2.5.1.9

Synonyms: ribE, ribB

Genomic Context

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Categories containing this gene/protein

Metabolism, Additional metabolic pathways, Biosynthesis of cofactors, Biosynthesis/ acquisition of riboflavin/ FAD

List of homologs in different organisms, belongs to COG0307 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, FMN-box, RibR regulon

Gene

Coordinates: 2,429,600  2,430,247

The protein

Catalyzed reaction/ biological activity

2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H⁺ --> 5-amino-6-(D-ribitylamino)uracil + riboflavin (according to UniProt)

Structure

1I8D (PDB) (from E. coli, 36% identity, 59% similarity) PubMed, , 1RVV (PDB) (the RibE)3-(RibH)60 lumazine synthase/riboflavin synthase complex) PubMed
P16440 (AlphaFold)

Localization

cytoplasm PubMed

Expression and Regulation

Operons

Genes: ribD-ribE-ribA-ribH-ribT

Description: PubMed

Regulation

expressed in the absence of FMN (FMN-box) PubMed
binding of RibR to the FMN-box riboswitch can enforce expression even in the presence of FMN PubMed
the FMN-box RNA is degraded by RNase Y PubMed

Regulatory mechanism

FMN-box: RNA switch, via FMN-box in the presence of FMN or FMNH₂, this is counter-acted upon binding of RibR, in FMN-box
RibR: antitermination, PubMed, in ribR regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

GP203 (erm), available in Jörg Stülke's lab
BKE23270 (ribE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTCCTGTAAACATGGTCA,  downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT
BKK23270 (ribE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATTCCTGTAAACATGGTCA,  downstream forward: _UP4_GGCTTTTAGAGAGGAAGATT

References

Reviews

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Original Publications

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