rapC

168

response regulator aspartate phosphatase, controls ComA activity

Locus: BSU_03770

Molecular weight: 45.47 kDa

Isoelectric point: 5.02

Protein length: 382 aa

Gene length: 1149 bp

Function: control of ComA activity

Product: response regulator aspartate phosphatase

Essential: no

Synonyms: rapC, yclL

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms

Gene

Coordinates: 428,831  429,979

The protein

Catalyzed reaction/ biological activity

• control of ComA activity PubMed

Protein family

• RAP family (according to UniProt)

Domains

• five tetratricopeptide repeats (according to UniProt)

Structure

• 4I9E (PDB) (RapF, 57% identity) PubMed
• P94415 (AlphaFold)

Effectors of protein activity

• binding of PhrC to RapC prevents it from binding ComA PubMed

Expression and Regulation

Operons

• Genes: rapC-phrC

  Description: PubMed

  Regulation

  • expressed at high cell density (ComA) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon
  • ComA: activation, (ComA) PubMed, in comA regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• MGNA-C065 (rapC::erm), available at the NBRP B. subtilis, Japan
• BKE03770 (rapC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTTCACCCTCTCCCA,  downstream forward: _UP4_CAAATACAAAGGAGTGAAGG
• BKK03770 (rapC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTTCACCCTCTCCCA,  downstream forward: _UP4_CAAATACAAAGGAGTGAAGG

References

Reviews

Loading

Original Publications

Loading