putP

putP
168

high affinity proline permease

locus
BSU_03220
Molecular weight
48.25 kDa
pI
8.62
Protein length
Gene length
function
proline uptake
product
high affinity proline permease
essential
no
synonyms
putP, ycgO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0591 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
347,150 → 348,571
Phenotypes of a mutant
no growth with proline as single source of carbon or nitrogen [Pubmed|21840319]
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The protein
Catalyzed reaction/ biological activity
uptake of proline coupled to the uptake of sodium ions
Protein family
[wiki|sodium:solute symporter (SSF) (TC 2.A.21) family] (according to UniProt)
Structure
[PDB|2XQ2] (from ''Vibrio parahaemolyticus'', 26% identity) [Pubmed|21131949]
[AF|P94392]
Paralogous protein(s)
[protein|6A1F759DAC09D364602460E5467E2761E97CE45C|opuE]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
description
(internal promoter) [Pubmed|21840319]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21840319,21964733,22139509], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Open in new tab

[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]

2024-02-12 01:55:36

Jstuelk

36

ba423fcf0254111de91945f6c4e9adef9d3e72a3

08A76DA5A75B56B9BB4705D2C33B96AA0C63A5C3

genes
[gene|1F548515402950572E7212901729B2480432D1B9|putB]-[gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]-[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]
description
[Pubmed|21840319,22139509]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR]: activation, [Pubmed|21840319,21964733,22139509], in [regulon|protein:C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR regulon]
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, displacement of [protein|C37D1CCBDE6DCEAB0AFACF8CBB3F733FE08324EE|putR] [Pubmed|21840319], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21840319,21964733,22139509], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
overexpressed in a [gene|64C6D783FF3F41C81B216F798A1DC8071345B1ED|pnpA] mutant [PubMed|14976255]
the [gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP] part of the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Open in new tab

[gene|1F548515402950572E7212901729B2480432D1B9|putB]→[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]

2024-02-24 23:35:08

Jstuelk

62

d991744c53f7fc7228712ba483de8c17cc05c88a

72CAEF916BFA077D3E9623FC66EECF026978B0D8

Biological materials
Mutant
MGNA-B994 (ycgO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1993 NBRP B. subtilis, Japan]
available in [wiki|Erhard Bremer]'s lab [Pubmed|24142252]
BKE03220 (Δ[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCGCCATATAAATAC,  downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
BKK03220 (Δ[gene|B3DF99B747D0A7472E3878920BF3CE7CE954B0BE|putP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCCCGCCATATAAATAC,  downstream forward: _UP4_TAAAGATCGAAAGGAGGAGG
References
21840319,21964733,22139509,24142252,21131949,26728461,26883633

B3DF99B747D0A7472E3878920BF3CE7CE954B0BE

Page visits: 5744

Time of last update: 2024-03-28 08:23:59

Author of last update: Melvin.boenninger