purB

168

adenylsuccinate lyase

Locus: BSU_06440

Molecular weight: 49.32 kDa

Isoelectric point: 5.82

Protein length: 431 aa

Gene length: 1296 bp

Function: purine biosynthesis

Product: adenylsuccinate lyase

Essential: no

E.C.: 4.3.2.2

Synonyms: purB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0015 (Galperin et al., 2021)

Gene

Coordinates: 700,232 701,527

Phenotypes of a mutant

• poor growth PubMed
• non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

• N⁶-(1,2-dicarboxyethyl)-AMP --> AMP + fumarate (according to UniProt)
• (2S)-2-[5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamido]succinate --> 5-amino-1-(5-phospho--D-ribosyl)imidazole-4-carboxamide + fumarate (according to UniProt)

Protein family

• lyase 1 family (according to UniProt)

Structure

• 1F1O (PDB)
• P12047 (AlphaFold)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD

  Description: PubMed

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

  Regulatory mechanism

  • G-box: RNA switch, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in purR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE06440 (purB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC
• BKK06440 (purB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCAGGTCTTGAATAACGTT, downstream forward: _UP4_TAGAAGAAGCTTTTAGCGGC

References

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