pucH

168

allantoinase

Locus: BSU_32410

Molecular weight: 48.17 kDa

Isoelectric point: 5.06

Protein length: 446 aa

Gene length: 1341 bp

Function: purine utilization

Product: allantoinase

Essential: no

E.C.: 3.5.2.5

Synonyms: pucH, yunH

Genomic Context

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Categories containing this gene/protein

Metabolism, Nucleotide metabolism, Utilization of nucleotides

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, PucR regulon

Gene

Coordinates: 3,327,247  3,328,587

The protein

Catalyzed reaction/ biological activity

allantoin + H₂O --> allantoate + H⁺ (according to UniProt)

Protein family

Metallo-dependent hydrolases superfamily (according to UniProt)

Structure

3HM7 (PDB) (from Bacillus halodurans, 44% identity, 65% similarity)
O32137 (AlphaFold)

Paralogous protein(s)

PyrC

Expression and Regulation

Operons

Genes: pucH

Description: PubMed

Regulation

induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

Regulatory mechanism

PucR: activation, PubMed, in pucR regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-A933 (yunH::erm), available at the NBRP B. subtilis, Japan
BKE32410 (pucH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
BKK32410 (pucH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGATCCCCCCATGTC,  downstream forward: _UP4_TAAGCCCGGCGGATTTTACC

References

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