proA

proA
168

glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis

locus
BSU_13130
Molecular weight
45.17 kDa
pI
5.1
Protein length
Gene length
function
biosynthesis of proline
product
glutamate-5-semialdehyde dehydrogenase
essential
no
ec
1.2.1.41
synonyms
proA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0014 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,379,605  1,380,852
Phenotypes of a mutant
auxotrophic for proline [Pubmed|21784929], this can be bypassed by suppressor mutations that allow overexpression of [protein|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD] (e.g. constitutive transcription activation by [protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR], generation of a [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]-dependent promoter upstream of [gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]) [pubmed|23869754]
not viable under conditions of osmotic stress (0.8 M NaCl) [pubmed|35783388,23869754], this can be bypassed by mutations that allow overexpression of the [gene|62678D45FA1CB1B6AB801FB9F574DDE10DEE068D|argC]-[gene|0FF858E44B3C557EE2C8B3749A458F152E1C535C|argJ]-[gene|8321DF6EE5B84CC9A4A5DF670E8611F4D61EBC7E|argB]-[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]-[gene|4202EAE5D0B2A718382092423DD99E35FDE81218|carA]-[gene|7E6386F1949A6F46E4332BAB8F803742EB076AF0|carB]-[gene|11E7D77EF9439F817C10BF5D9671C5AE66AF06DC|argF] operon resulting from inactivation of [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] or the [protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] binding site in front of the operon) [pubmed|35783388]
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The protein
Catalyzed reaction/ biological activity
L-glutamate 5-semialdehyde + NADP+ + phosphate --> H+ + L-glutamyl 5-phosphate + NADPH (according to UniProt)
Protein family
gamma-glutamyl phosphate reductase family (single member, according to UniProt)
Structure
[PDB|1O20] (from ''Thermotoga maritima'', 46% identity, 67% similarity) [Pubmed|14705032]
[AF|P39821]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]-[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]
description
[Pubmed|21233158]
regulation
induced by proline limitation ([wiki|T-box]) [Pubmed|21233158]
expression requires functional [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21233158], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
[protein|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA] is absent from the membrane proteome of a '[protein|A01637E1BFABA20A2923BA85A25CCD8A0D665F78|bdbC]-[protein|CED818CEF5B573A40F382157375E60C0B331F098|bdbD]' mutant [PubMed|22540663]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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[gene|5592C5163CF024289B850916516E2C8C7AD43136|proB]→[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]

2024-03-16 19:27:08

Jstuelk

55

a498fd63a6f2f5e81db95fe3ac7abe7368864c98

75EDCA3FF56BE0FC681BC2D3D6B71BE06F829994

Biological materials
Mutant
GP4087 (Δ[gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]::''phleo'') available in [wiki|Jörg Stülke]'s lab
BKE13130 ([gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACTTCACTCATTTTTCCGC,  downstream forward: _UP4_TAGCGGGGTAATGTTCAATG
BKK13130 ([gene|1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9|proA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_AACTTCACTCATTTTTCCGC,  downstream forward: _UP4_TAGCGGGGTAATGTTCAATG
References
Reviews
25367752
Original Publications
19258532,21233158,22540663,21784929,17562291,23869754,25344233,28752945,30355672,35783388

1629A875FCCA0C0EB1C959ED31BB6B3CFA839BF9

Page visits: 3487

Time of last update: 2024-03-29 04:29:24

Author of last update: Robert.warneke