prfA

168

peptide chain release factor 1, recognize stop codon and hydrolyze the completed protein, RF1 recognizes UAA and UAG

locus: BSU_37010

Molecular weight: 40.07 kDa

pI: 4.82

Protein length: 356 aa

Gene length: 1071 bp

function: translation

product: peptide chain release factor 1

essential: yes

synonyms: prfA

List of homologs in different organisms, belongs to COG0216 (Galperin et al., 2021)

Gene

Coordinates: 3,797,085  3,798,155

Phenotypes of a mutant

• essential [Pubmed|12682299]

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The protein

Catalyzed reaction/ biological activity

• binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfA is highly specific for a U in the first stop-codon position and recognizes UAA and UAG [pubmed|11158343]

Protein family

• prokaryotic/mitochondrial release factor family (with [protein|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB], according to UniProt)

Structure

• [PDB|1ZBT] (from ''Streptococcus mutans'', 58% identity)
• [AF|P45872]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]-[gene|CF959D90A9B8F83E40E7B81CBFB00B5EC09EC0DD|prmC]-[gene|B56B38A1CCC0A73FFD9F3F4C9942356E6D818D2D|yfkF]

  description: [Pubmed|22383849]

• genes: [gene|59E6468FD891F5271F38543515867B0B4625768D|prfA]

  description: [Pubmed|22383849]

References

Reviews

• 11158343

Original Publications

• 12237459,20512119,25217634,33900014