ppsB

168

plipastatin synthetase

Locus: BSU_18330

Molecular weight: 289.84 kDa

Isoelectric point: 5.06

Protein length: 2560 aa

Gene length: 7683 bp

Function: production of the antibacterial compound plipastatin

Product: plipastatin synthetase

Essential: no

Synonyms: ppsB

Genomic Context

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List of homologs in different organisms, belongs to COG3433 (Galperin et al., 2021)

Gene

Coordinates: 1,982,565 → 1,990,247

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

• 2 Carrier domains (aa 965-1040, aa 2006-2080) (according to UniProt)

Structure

• 2VSQ (PDB) (termination module of SrfAC, 38% identity) PubMed
• P39846 (AlphaFold)

Expression and Regulation

Operons

• Genes: ppsA-ppsB-ppsC-ppsD-ppsE

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• BKE18330 (ΔppsB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTCCACACACCTTT,  downstream forward: _UP4_AAACTATAGGAGAGGAGATT
• BKK18330 (ΔppsB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTCCACACACCTTT,  downstream forward: _UP4_AAACTATAGGAGAGGAGATT

References

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