pksR

168

polyketide synthase

Locus: BSU_17220

Molecular weight: 284.73 kDa

Isoelectric point: 5.44

Protein length: 2543 aa

Gene length: 7632 bp

Function: polyketide synthesis

Product: polyketide synthase

Essential: no

Synonyms: pksR

Genomic Context

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List of homologs in different organisms, belongs to COG3321 (Galperin et al., 2021)

Gene

Coordinates: 1,850,890  1,858,521

The protein

Domains

• 3 Carrier domains (aa 376-452, aa 1407-1485, aa 2134-2208) (according to UniProt)

Structure

• 4U3V (PDB) (enoyl-isomerase domain, aa 1124-1395) PubMed
• O31784 (AlphaFold)

Expression and Regulation

Operons

• Genes: pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

  Regulation

  • expressed during the transition from growth to stationary phase (AbrB, CodY) PubMed
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • CodY: activation, PubMed, in codY regulon

  Additional information

  • this is a very large operon comprising about 75 kb

• Genes: pksB-pksC-pksD-pksE-acpK-pksF-pksG-pksH-pksI-pksJ-pksL-pksM-pksN-pksR

  Description: PubMed

Biological materials

Mutant

• MGNA-A018 (pksR::erm), available at the NBRP B. subtilis, Japan
• BKE17220 (pksR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCAGCATTGGCGTTTCCC,  downstream forward: _UP4_TGAGAAAACACAAACGCCCC
• BKK17220 (pksR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTCAGCATTGGCGTTTCCC,  downstream forward: _UP4_TGAGAAAACACAAACGCCCC

References

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