pgdS

168

gamma-DL-glutamyl hydrolase

Locus: BSU_35860

Molecular weight: 45.09 kDa

Isoelectric point: 8.52

Protein length: 413 aa

Gene length: 1242 bp

Function: polyglutamic acid degradation

Product: gamma-DL-glutamyl hydrolase

Essential: no

Synonyms: pgdS, ywtD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)

Gene

Coordinates: 3,696,257  3,697,498

The protein

Protein family

• Peptidase C40 family (according to UniProt)

Domains

• three NlpC/P60 domains (only the central domain is active) PubMed

Structure

• 4HPE (PDB) (from Clostridium difficile, 39% identity)
• P96740 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: pgdS

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• MGNA-A545 (ywtD::erm), available at the NBRP B. subtilis, Japan
• BKE35860 (pgdS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATTATCTCCTCCT,  downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT
• BKK35860 (pgdS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTTATTATCTCCTCCT,  downstream forward: _UP4_TAAAAGAAAAAGACTCCAAT

LacZ fusion

• available in Daniel Kearns's lab PubMed

References

Reviews

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Original Publications

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