penP

168

beta-lactamase

locus: BSU_18800

Molecular weight: 33.29 kDa

pI: 9.21

Protein length: 306 aa

Gene length: 921 bp

function: resistance to beta-lactam antibiotics

product: beta-lactamase

essential: no

ec: 3.5.2.6

synonyms: penP

List of homologs in different organisms, belongs to COG2367 (Galperin et al., 2021)

Gene

Coordinates: 2,048,533  2,049,453

The protein

Catalyzed reaction/ biological activity

• β-lactam + H₂O --> substituted β-amino acid (according to UniProt)

Protein family

• [wiki|beta-lactamase family] (according to UniProt)
• class-A beta-lactamase family (single member, according to UniProt)

Structure

• [PDB|6NI1]
• [AF|P39824]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|713BAB7190E1F86C55103049B29072F00E0DFFB3|penP]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE18800 ([gene|713BAB7190E1F86C55103049B29072F00E0DFFB3|penP]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGGCCTCCTCATTCAA,  downstream forward: _UP4_TAATATGTTTAGCCTTTTGC
• BKK18800 ([gene|713BAB7190E1F86C55103049B29072F00E0DFFB3|penP]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCAGGCCTCCTCATTCAA,  downstream forward: _UP4_TAATATGTTTAGCCTTTTGC

References

• 18957862,30637927