pbuG

168

hypoxanthin/ guanine permease

locus: BSU_06370

Molecular weight: 46.03 kDa

pI: 8.81

Protein length: 440 aa

Gene length: 1323 bp

function: hypoxanthine and guanine uptake

product: hypoxanthin/ guanine permease

essential: no

ec: null

synonyms: pbuG, yebB

List of homologs in different organisms, belongs to COG2252 (Galperin et al., 2021)

Gene

Coordinates: 694,662  695,984

The protein

Protein family

• [wiki|xanthine/uracil permease family] (according to UniProt)

Structure

• [PDB|8IRL] (from Arabidopsis thaliana, 32% identity) [pubmed|38172575]
• [PDB|2EEW] (the [gene|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG] riboswitch, U47c mutant, bound to hypoxanthine)
• [AF|O34987]

Paralogous protein(s)

• [protein|62AC66725C55B9E5FBBEB4EC43A88F7081B74D5C|pbuO]

[wiki|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG]

  description: [Pubmed|12923093]

  regulation

  • repressed by hypoxanthine and guanine, [protein|search|PurR]-independent, ([protein|search|G-box])[Pubmed|12923093]

  regulatory mechanism

  • [protein|A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR]: RNA switch, (molecular inducer: PRPP) [Pubmed|2536750], in [regulon|protein:A8C82F5825DE0921DA6B4ACDE0CC31EC7749273C|purR regulon]
  • G-box: termination, ([protein|C245476CE35028167510F67153F392D91447553F|mgtE riboswitch]) [Pubmed|12923093], in [regulon|other_regulator:G-box|G-box]

Biological materials

Mutant

• BKE06370 ([gene|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCTATTTGACTCCCTTC,  downstream forward: _UP4_TAAGGAATGAAAAACCAGCT
• BKK06370 ([gene|41DDD3AAFE89D0F52AB5ABD08CEE30A1767FFD7A|pbuG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGCTATTTGACTCCCTTC,  downstream forward: _UP4_TAAGGAATGAAAAACCAGCT

References

• 11591660,12923093,11591660,18763711,12923093,12787499,35622174,38172575