pbpE

168

Class C penicillin-binding protein PBP 4*

Locus: BSU_34440

Molecular weight: 51.27 kDa

Isoelectric point: 4.84

Protein length: 451 aa

Gene length: 1356 bp

Function: endopeptidase

Product: penicillin-binding protein PBP 4*

Essential: no

Synonyms: pbpE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)

Gene

Coordinates: 3,534,118 3,535,473

The protein

Protein family

• beta-lactamase family (according to UniProt)

Structure

• 3TG9 (PDB) (from Bacillus halodurans, 27% identity, 59% similarity)
• P32959 (AlphaFold)

Localization

• cell membrane (according to UniProt)
• distinct foci and bands at cell periphery PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pbpE-racX

  Description: PubMed

  Regulation

  • induced by cell wall stress (SigW) PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• BKE34440 (pbpE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA
• BKK34440 (pbpE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCTCCACCTCCCATAT, downstream forward: _UP4_ACGAAGTAGAAAGGAATGAA

GFP fusion

• 3106 trpC2 pbpE::pSG5046 (cat P_xyl-gfpa-pbpE1-761) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

References

Reviews

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Original Publications

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