patB

168

cystathione-beta-lyase

locus: BSU_31440

Molecular weight: 42.63 kDa

pI: 5.44

Protein length: 387 aa

Gene length: 1164 bp

function: biosynthesis of methionine

product: cystathione-beta-lyase

essential: no

ec: 4.4.1.8

synonyms: patB

List of homologs in different organisms, belongs to COG1168 (Galperin et al., 2021)

Gene

Coordinates: 3,228,778  3,229,941

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The protein

Catalyzed reaction/ biological activity

• H₂O + L,L-cystathionine --> L-homocysteine + NH₄⁺ + pyruvate (according to UniProt)

Protein family

• [wiki|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|3T32] (from ''B. anthracis'', 46% identity, 75% similarity)
• [AF|Q08432]

Expression and Regulation

Operons

• genes: [gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]

  description: [Pubmed|9274030]

Biological materials

Mutant

• 1A952 ( ''patB''::''cat''), [Pubmed|15760717], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A952&Search=1A952 BGSC]
• BKE31440 ([gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTACCCCCTCATG,  downstream forward: _UP4_TAAATATAGCTGTAAACGCC
• BKK31440 ([gene|11B54D438CDD2EAA4A87A6B99851A3B123EA4A1F|patB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31440 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCTGTTACCCCCTCATG,  downstream forward: _UP4_TAAATATAGCTGTAAACGCC

References

• 9274030,15760717