panC

168

pantothenate synthase

locus: BSU_22420

Molecular weight: 31.81 kDa

pI: 4.63

Protein length: 286 aa

Gene length: 861 bp

function: biosynthesis of coenzyme A

product: pantothenate synthase

essential: quasi

ec: 6.3.2.1

synonyms: panC

List of homologs in different organisms, belongs to COG0414 (Galperin et al., 2021)

Gene

Coordinates: 2,352,977  2,353,837

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
• quasi-essential, the mutant is auxotrophic for pantothenate [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]
• the mutant is viable if it acquires mutations ([gene|50930C56C27D22715620A350220E3C56ADB41020|cymR] or [gene|D1FC976597E5583E40A4ED7234FBCA743AB01354|cysK]) that allow constitutive expression of [gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN] for the uptake of cysteinopantetheine [https://www.biorxiv.org/content/10.1101/2024.04.17.589903v1.full.pdf bioRxiv]

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The protein

Catalyzed reaction/ biological activity

• (R)-pantoate + ATP + β-alanine --> (R)-pantothenate + AMP + diphosphate + H⁺ (according to UniProt)

Protein family

• pantothenate synthetase family (single member, according to UniProt)

Structure

• [PDB|2EJC] (from ''Thermotoga maritima'', 50% identity, 66% similarity)
• [AF|P52998]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A0001681244113B808757281C1B70C0C94F28A27|panB]-[gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]-[gene|EE712C1EC9E9C371847C83F7D75C189B276CD4B6|panD]-[gene|03BFD8AD2E3D95858752F46366F6C2028D709029|dinG]-[gene|08F907C6F47BDEB4DC9A9F136CDC6A4063AC0140|ypmA]-[gene|D3629004F3B54D11566AE15227174F9EB7784201|tseB]-[gene|AE802E1D4F827DED3A5D953C7D034E6A3554C45F|aspB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE22420 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGAAATATCAGTAATCTGTC,  downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
• BKK22420 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGAAATATCAGTAATCTGTC,  downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
• GP3379 ([gene|8BE55434677F4EC3D6766C7163496CB1B659F57F|panC]::neo trpC2), available in [wiki|Jörg Stülke]'s lab, use GP4402

Expression vectors

• pGP3723: expression of ''panC'' (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab

References

• 35153321