oxdC

168

oxalate decarboxylase

locus: BSU_33240

Molecular weight: 43.41 kDa

pI: 5.1

Protein length: 385 aa

Gene length: 1158 bp

function: unknown

product: oxalate decarboxylase

essential: no

ec: 4.1.1.2

synonyms: oxdC, yvrK

List of homologs in different organisms, belongs to COG2140 (Galperin et al., 2021)

Gene

Coordinates: 3,410,466  3,411,623

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The protein

Catalyzed reaction/ biological activity

• H⁺ + oxalate --> CO₂ + formate (according to UniProt)

[wiki|Cofactors]

• requires two Mn(II) centers for activity [Pubmed|24444454,19473032]

Structure

• [PDB|1L3J] [pubmed|14871895]
• [AF|O34714]

Modification

• phosphorylated on Arg-12 [Pubmed|22517742]

Paralogous protein(s)

• [protein|1568DD457FDD482B3C14954A95F62BE338CBD15D|oxdD]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|9793E952C985BA268AC28C907900A03F4E8A3253|oxdC]-[gene|07C35D58F6AF97F8E6BB75CF8E8B811BAFAB201B|rsiO]

  description: [Pubmed|19047353]

  regulation

  • induced by acidic growth conditions [Pubmed|19047353]

  sigma factors

  • [protein|6359025A73C0BD313F5BCC6F56FE88B820902AAA|sigO]: sigma factor, [Pubmed|19047353], in [regulon|protein:6359025A73C0BD313F5BCC6F56FE88B820902AAA|sigO regulon]
  • [protein|AE8D4F07EFB17DA727D80229C3E72075928AA352|rsoA]: sigma factor, in [regulon|protein:AE8D4F07EFB17DA727D80229C3E72075928AA352|rsoA regulon]

Biological materials

Mutant

• MGNA-B053 (yvrK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1052 NBRP B. subtilis, Japan]
• BKE33240 ([gene|9793E952C985BA268AC28C907900A03F4E8A3253|oxdC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAATGTTTCCTCCTTA,  downstream forward: _UP4_TAAAAGACTTGCGGCTTGCA
• BKK33240 ([gene|9793E952C985BA268AC28C907900A03F4E8A3253|oxdC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAAATGTTTCCTCCTTA,  downstream forward: _UP4_TAAAAGACTTGCGGCTTGCA

labs

• [wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Reviews

• 20464388

Original Publications

• 12056897,18573182,17269748,14871895,10960116,19047353,19473032,11546787,15583401,21264418,21782782,22404040,23734819,25526893,21277974,22517742,24444454,25186982,26641915,26744902,27797181,14871895,29620872,30806021,34097877,36482787