nupO

168

ABC transporter for guanosine (ATP-binding protein)

Locus: BSU_31550

Molecular weight: 56.13 kDa

Isoelectric point: 7.5

Protein length: 510 aa

Gene length: 1533 bp

Function: uptake of guanosine

Product: ABC transporter for guanosine

Essential: no

Synonyms: nupO, yufO

Genomic Context

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List of homologs in different organisms, belongs to COG3845 (Galperin et al., 2021)

Gene

Coordinates: 3,241,085  3,242,617

Phenotypes of a mutant

• a ''nupO nupG'' double mutant is unable to utilize externally provided guanosine PubMed
• defective in the the ability to support the growth of Synechococcus leopoliensis CCAP1405/1 on agar media PubMed

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• 2 ABC transporter domains (aa 5-240, aa 257-501) (according to UniProt)

Structure

• 5XJY (PDB) (human protein, 30% identity) PubMed
• O05253 (AlphaFold)

Paralogous protein(s)

• RbsA

Localization

• associated to the membrane (via NupP-NupQ) PubMed

Expression and Regulation

Operons

• Genes: nupN-nupO-nupP-nupQ

  Description: PubMed

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B554 (yufO::erm), available at the NBRP B. subtilis, Japan
• BKE31550 (nupO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTGACCGCTCCTTT,  downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA
• BKK31550 (nupO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATTGACCGCTCCTTT,  downstream forward: _UP4_ACGCAAAAGGAAGCGGGGAA

References

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