nucA

168

membrane-associated nuclease, catalyzes DNA cleavage during transformation

locus: BSU_03430

Molecular weight: 16.12 kDa

pI: 4.68

Protein length: 147 aa

Gene length: 444 bp

function: genetic transformation, DNA uptake

product: membrane-associated nuclease

essential: no

synonyms: nucA, comI

List of homologs in different organisms

Gene

Coordinates: 372,154  372,597

The protein

Structure

• [PDB|5OMT] ([protein|search|NucB ]from B. licheniformis, 64% identity, aa 40 - 147) [pubmed|29165717]
• [AF|P12667]

[wiki|Localization]

• integral membrane protein [Pubmed|11359569]

Expression and Regulation

Operons

• genes: [gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]-[gene|3A54C644DD1CCA5C08CB9C5CD648D44889E68D23|nin]

  description: [Pubmed|11359569]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|7746143], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]

Biological materials

Mutant

• BKE03430 ([gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT,  downstream forward: _UP4_TAAACAGTACATAGAGGAGG
• BKK03430 ([gene|99581CC6AFFA2D1392748B4D9C99DEF8B7601F75|nucA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT,  downstream forward: _UP4_TAAACAGTACATAGAGGAGG

References

• 11814663,11359569,7746143,29165717,34126763,39375363