nucA

168

membrane-associated nuclease, catalyzes DNA cleavage during transformation

Locus: BSU_03430

Molecular weight: 16.12 kDa

Isoelectric point: 4.68

Protein length: 147 aa

Gene length: 444 bp

Function: genetic transformation, DNA uptake

Product: membrane-associated nuclease

Essential: no

Synonyms: nucA, comI

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 372,154  372,597

The protein

Structure

• 5OMT (PDB) (NucB from B. licheniformis, 64% identity, aa 40 - 147) PubMed
• P12667 (AlphaFold)

Localization

• integral membrane protein PubMed

Expression and Regulation

Operons

• Genes: nucA-nin

  Description: PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

Biological materials

Mutant

• BKE03430 (nucA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT,  downstream forward: _UP4_TAAACAGTACATAGAGGAGG
• BKK03430 (nucA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATGTTCACCTCCCGCTTTT,  downstream forward: _UP4_TAAACAGTACATAGAGGAGG

References

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