noc

noc
168

DNA-binding protein, spatial regulator of [wiki|cell division] to protect the nucleoid, and timing device with an important role in the coordination of chromosome segregation and [wiki|cell division], Z ring placement, coordination of early steps in DNA replication with establishment of a medial division site

locus
BSU_40990
Molecular weight
32.64 kDa
pI
5.81
Protein length
Gene length
function
control of [wiki|cell division]
product
effector of nucleoid occlusion
essential
no
synonyms
noc, yyaA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1475 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,207,897  4,208,748
Phenotypes of a mutant
a [gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
severe growth defect of a [gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant at increased temperature (37°C or above) due to a maturation defect of the Z-ring for [wiki|cell division], this can be suppressed by any mutation affecting the [protein|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-[protein|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] proteolytic system due to [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]-mediated increased expression of [protein|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] [pubmed|34181483]
a [gene|C5F67C3ACB06A5029B4AE69A065BC55622372404|refZ] [gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc] double mutant is defective in [wiki|cell division] and exhibits reduced sporulation [pubmed|35506695]
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The protein
Catalyzed reaction/ biological activity
binds specific sites on the chromosome [Pubmed|19494834]
prevents Z ring assembly over the bacterial nucleoid and helps fine tune the assembly of the Z ring at midcell during the cell cycle [pubmed|31152469]
Protein family
ParB family (with [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB], according to UniProt)
Structure
[PDB|7OL9] (C-terminally truncated Noc complexed to the Noc-binding site) [pubmed|36841481]
[PDB|1VZ0] ([protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] from Thermus thermophilus, 48% from aa 30 ... 219) [pubmed|15228524]
[AF|P37524]
Paralogous protein(s)
[protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB]
[wiki|Localization]
associated to the cell membrane, membrane association is triggered by binding of CTP [Pubmed|34270916,25568309]
Expression and Regulation
Operons
genes
[gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]-[gene|7809B4F5B9172EBB6C91BDE7B673FB08E82763FF|gidA]-[gene|CCB7EE1D049585979303148FBDE0D42549E648DE|gidB]-[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]
description
[Pubmed|11807071]
regulation
constitutively expressed [Pubmed|23701187]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]: repression, [Pubmed|26020636], in [regulon|protein:6740108089F13116F200C15F35C2E7561E990FEB|dnaA regulon]
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[gene|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF]→[gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]

2024-03-25 19:19:42

ghost

101

97e0c96ef02da18e76bff0ad52ca51aad0c040d6

02D91CE1EAA3DC9BAA9E45D09CC54A749E1D1DD6

Biological materials
Mutant
MGNA-B874 (yyaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1873 NBRP B. subtilis, Japan]
BKE40990 ([gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACCTACACCACCTTT,  downstream forward: _UP4_TAGAAGCTCTCCTGAAAAGC
BKK40990 ([gene|559DEDC9887B811EF80994526256ADC48BA51CE3|noc]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTACCTACACCACCTTT,  downstream forward: _UP4_TAGAAGCTCTCCTGAAAAGC
GFP fusion
available in the [wiki|Jeff Errington] lab
labs
Jeff Errington, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington Homepage]
David Rudner, Harvard, USA [http://rudnerlab.med.harvard.edu/ Homepage]
References
Reviews
19680248,19884039,25460802,26706151,28500529,31254421
Original Publications
16549676,11807071,19494834,15210112,11948146,22457634,23701187,24097947,25568309,26020636,28723932,15228524,31152469,34181483,34270916,35506695,36841481

559DEDC9887B811EF80994526256ADC48BA51CE3

Page visits: 4873

Time of last update: 2024-03-28 17:16:32

Author of last update: Jstuelk