nifS

168

cysteine desulfurase, required for NAD biosynthesis

Locus: BSU_27880

Molecular weight: 43.63 kDa

Isoelectric point: 6.43

Protein length: 395 aa

Gene length: 1188 bp

Function: NAD biosynthesis

Product: cysteine desulfurase

Essential: no

E.C.: 2.8.1.7

Synonyms: nifS, iscS

Genomic Context

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List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)

Gene

Coordinates: 2,849,569  2,850,756

The protein

Catalyzed reaction/ biological activity

• [sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)

Protein family

• Class-V pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• pyridoxalphosphate PubMed

Structure

• 4R5F (PDB) (from Archaeoglobus fulgidus, 34% identity) PubMed
• P38033 (AlphaFold)

Paralogous protein(s)

• YrvO, YcbU, NifZ, SufS

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: nifS-nadR

  Description: PubMed

  Regulation

  • repressed in the presence of nicotinic acid (NadR) PubMed

  Regulatory mechanism

  • NadR: repression, PubMed, in nadR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE27880 (nifS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC,  downstream forward: _UP4_ATATTGACCGAAGAATTAAA
• BKK27880 (nifS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC,  downstream forward: _UP4_ATATTGACCGAAGAATTAAA

References

Reviews

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Original Publications

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