nifS
168
cysteine desulfurase, required for NAD biosynthesis
locus
BSU_27880
Molecular weight
43.63 kDa
pI
6.43
function
NAD biosynthesis
product
cysteine desulfurase
essential
no
ec
2.8.1.7
synonyms
nifS, iscS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1104 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,849,569 2,850,756
The protein
Catalyzed reaction/ biological activity
[sulfur carrier]-H + L-cysteine --> [sulfur carrier]-SH + L-alanine (according to UniProt)
Protein family
[wiki|Class-V pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
pyridoxalphosphate [Pubmed|25447671]
Structure
[PDB|4R5F] (from Archaeoglobus fulgidus, 34% identity) [pubmed|25447670]
[AF|P38033]
Paralogous protein(s)
[protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO], [protein|5424A936643C90B3DA2CB60FD0FD42A64F2BAF09|ycbU], [protein|741DCFFBB8FF6A4E357EE5110EBE24412DE34244|nifZ], [protein|88ACE6B79534338F7C72F107B35A0B2384007088|sufS]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]-[gene|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]
description
[Pubmed|8444804]
regulation
repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|8444804,16199587]
regulatory mechanism
[protein|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]: repression, [Pubmed|8444804,16199587], in [regulon|protein:510DE075EA964D22C42DEB171509F6CDA600A402|nadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8444804], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE27880 ([gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC, downstream forward: _UP4_ATATTGACCGAAGAATTAAA
BKK27880 ([gene|A6746AE1B92A0CDCCB22B57B6DF78094BB0223A8|nifS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGGTCTTCCTCCATCC, downstream forward: _UP4_ATATTGACCGAAGAATTAAA
References
Reviews
Original Publications
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Time of last update: 2025-05-09 15:00:55
Author of last update: Jstuelk