nicK

168

DNA relaxase, similar to transposon protein

Locus: BSU_04870

Molecular weight: 40.78 kDa

Isoelectric point: 5.13

Protein length: 352 aa

Gene length: 1059 bp

Function: conjugation of ICE BS1

Product: DNA relaxase, similar to transposon protein

Essential: no

E.C.: null

Synonyms: nicK, ydcR

Genomic Context

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List of homologs in different organisms, belongs to COG2946 (Galperin et al., 2021)

Gene

Coordinates: 534,773  535,831

The protein

Protein family

• plasmid replication initiation factor family (single member, according to UniProt)

Structure

• 4CIJ (PDB) (from Geobacillus stearothermophilus, corresponds to aa 26 ... 289, 33% identity) PubMed
• P96635 (AlphaFold)

Expression and Regulation

Operons

• Genes: xis-ydzL-devI-ydcP-ydcQ-nicK-S172-ramS-ramT-ramA-conB-conC-conD-conE-yddF-conG-cwlT-yddI-yddJ

  Description: PubMed

  Regulatory mechanism

  • ImmR: repression, PubMed, in immR regulon

Biological materials

Mutant

• BKE04870 (nicK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTCATCCATTCGCTTCGCT,  downstream forward: _UP4_TAAAACCAAGCTGCTAAACC
• BKK04870 (nicK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTCATCCATTCGCTTCGCT,  downstream forward: _UP4_TAAAACCAAGCTGCTAAACC

References

Reviews

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Original Publications

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