nfrA

168

[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein

locus: BSU_38110

Molecular weight: 28.17 kDa

pI: 5.73

Protein length: 249 aa

Gene length: 750 bp

function: unknown

product: FMN-containing NADPH-linked nitro/flavin reductase

essential: no

synonyms: nfrA, ywcG, ipa-43d

List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)

Gene

Coordinates: 3,911,477  3,912,226

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The protein

Catalyzed reaction/ biological activity

• possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD( ) degrading activity leading to free nicotinamide [Pubmed|20727352]
• FMNH₂ + NADP⁺ --> FMN + 2 H⁺ + NADPH (according to UniProt)

Protein family

• flavin oxidoreductase frp family (with [protein|BA6C61C488659D5F1B5237BA817A8A5AC6D0E988|ycnD], according to UniProt)

[wiki|Cofactors]

• FMN or FAD [Pubmed|21635694]

Structure

• [PDB|3N2S] [Pubmed|20727352]
• [AF|P39605]

Expression and Regulation

Operons

• genes: [gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]-[gene|A1DCFF8D225C87197104742E72A0D4F238BC30EB|ywcH]

  description: [Pubmed|10913069]

  regulation

  • repressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|spx]) [pubmed|29271514]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|29271514,12642660,19575568], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10913069], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|9535080], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]

Biological materials

Mutant

• MGNA-B232 (ywcG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1231 NBRP B. subtilis, Japan]
• BKE38110 ([gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT,  downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT
• BKK38110 ([gene|5B95F7CFEFCB954FCBC11087F030A1D777F34B7A|nfrA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCACAAAAACCTCCTTAT,  downstream forward: _UP4_TAAAGCAAACCTCTCCGTCT

References

• 9535080,16847875,10913069,12642660,14651647,20727352,9836433,24847778,29271514